BBa_J100303

BBa_J100303 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100303

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emilie Uffman

Date created: 2016-09-07 11:00:00

Date modified: 2016-09-08 01:31:49

PmanP

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100303_sequence (Version 1)

Description

The PmanP promoter, when induced by mannose, increases the beta-galactosidase activity of bacteria, which thus increases the expression of lacZ by four to seven-fold. In this experiment, we are using a 0.2% concentration of mannose in order to test the induction of transcription.

Notes

During the design it was found that several derivatives of the PmanP promoter did not induce beta-galactosidase, it was then decided to cut out those derivatives in order to use a sequence that induced beta-galactosidase while staying under the 60 base pair maximum requirement.

Source

Bacillus subtilis
Sun, Tianqi; Altenbuchner, Josef. ???Charaterization of a Mannose Utilization System in Bacillus subtilis.??? Journal of Bacteriology, vol. 192, No. 8, 2010, pp. 2128-2139.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10 box	39,43	non_covalent_binding_site, feature/binding
-35 box	15,20	non_covalent_binding_site, feature/binding

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100303/1

Member of these Collections

• iGEM Parts Registry