Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-10 11:48:04
repClone Red (J100205) with wt TetR promoter (R0040)
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_J100306_sequence (Version 1) |
Description
repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system.Notes
None.Source
This part can be constructed by ligating the TetR promoter into repClone (J100205) following BsaI digestion.| Sequence Annotation | Location | Component / Role(s) |
| tetR Repressor C0041 BD24 P5 Promoter (for tetR) GFP Optimized Sequence B0034 BsaI Sticky End TetR Repressible Promoter (B0040) BsaI Sticky End BD18 RFP | 1,639 640,727 728,763 764,1483 1490,1501 1512,1515 1516,1569 1570,1573 1574,1661 1659,2339 | CDS feature/protein feature/rbs ribosome_entry_site feature/promoter promoter feature/protein CDS ribosome_entry_site feature/rbs sequence_feature feature/misc promoter feature/promoter sequence_feature feature/misc ribosome_entry_site feature/rbs feature/protein CDS |