Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2016-12-02 12:00:00
Date modified: 2016-12-03 01:43:32
Scrambled TetR repressible promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100311_sequence (Version 1) |
Description
We scrambled the TetR binding regions of the TetR repressible promoter (R0040). The scrambled promoter is not functional, whether or not aTc is present to free the binding sites. We will use it as a negative control when using repClone (J100205) to test the effects of modifications to the promoter.Notes
We were cautious to scramble only those bases involved in TetR binding, while keeping other bases the same in order to maintain as best we can the spacing and structure of the original promoter.Source
The original sequence is the TetR repressible promoter, R0040. Binding regions were identified from Orth P, et al., "Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system." DOI: 10.1038/73324| Sequence Annotation | Location | Component / Role(s) |
| 5' TetR binding site 3' TetR binding site | 4,16 29,41 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |