Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2012-01-10 12:00:00
Date modified: 2015-08-31 04:08:24
Lux Operon Vibrio fischeri from part K325909
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J10064_sequence (Version 1) |
Description
This page described the lux operon from Vibrio fischeri. To relieve LuxR control we placed Lux C, D, A, B, E under a promoter of your choice. A more complete description can be found on the [Cambridge iGEM 2010 website.This Bacterial lux operons encodes five enzymes involved in the light-generating pathway. LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal. The exact function of LuxG is unknown, and it appears to be non-essential for light emission, but its presence increases light output.
In nature, the lux genes appear to be repressed by the nucleoid protein, H-NS, and occur under quorum sensing control. We removed the natural quorum sensing control to facilitate use of the part in biosensors under different regulatory inputs.
As of October 2010 Cambridge believe this is the first and only BioBrick to emit light in normal E. coli strains without the addition of any external substrate.
Notes
removed pBad to make it more usable for different purposesSource
from K325909 with pBad removed, synthesized de novo| Sequence Annotation | Location | Component / Role(s) |
| endogenous #1 luxC endogenous #2 luxD endogenous #3 luxA endogenous #4 luxB endogenous #5 luxE endogenous #6? uxG | 1,10 11,1450 1451,1462 1465,2369 2404,2413 2419,3483 3507,3518 3522,4502 4565,4573 4577,5713 5705,5709 5717,6427 | ribosome_entry_site feature/rbs CDS feature/cds feature/rbs ribosome_entry_site feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS |