Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2012-01-10 12:00:00
Date modified: 2015-08-31 04:08:24
luciferase + LRE + BD18 original RBS removed
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J10068_sequence (Version 1) |
Description
Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)The original part (K325229) was used as the template. We have removed all aspects of the pBad promoter and AraC regulatory DNA as well as the original first RBS. This part now starts with BD18 (J119024).
K325229 is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.
Notes
removed RBS to allow C-dog system to work.Source
from registry parts, synthesized de novo| Sequence Annotation | Location | Component / Role(s) |
| GGA prefix sticky word AATG ATG from C-dog bicistron double stop luciferase generating enzyme ATG green luciferase double stop BBa suffix | 1,17 15,17 942,947 15,941 971,973 971,2620 2615,2620 2621,2641 | sequence_feature feature/dna start_codon feature/start stop_codon feature/stop CDS feature/protein feature/start start_codon feature/protein CDS feature/stop stop_codon feature/misc sequence_feature |