BBa_J102000

BBa_J102000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J102000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Pettigrew

Date created: 2010-07-01 11:00:00

Date modified: 2015-08-31 04:08:24

RBS + GFP with LVA

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_J102000_sequence (Version 1)

Description

A standard ribosome binding site with an efficiency of 1.0 preceding a protein-coding sequence which codes for Green Fluorescent Protein with an LVA (degradation) tag.

Notes

Used GFP with LVA tag so that response of this parts (when used with a valid promoter and terminator) could be characterized more easily.
Used high-efficiency, standard RBS so that there will be a larger dynamic range of GFP expression, which can be controlled at the gene level.

Source

Source is using the 3A assembly method of biobrick construction with the parts B0034 and K145015, both obtained from the 2010 iGEM DNA Distribution.

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_K145015	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_K145015	19,777	GFP

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J102000/1

Member of these Collections

• iGEM Parts Registry