BBa_J107010

BBa_J107010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J107010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lorenzo Pasotti

Date created: 2010-05-31 11:00:00

Date modified: 2015-08-31 04:08:24

R0011 measurement system (with RFP)

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_J107010_sequence (Version 1)

Description

This part can be used to characterize BBa_R0011 hybrid promoter using GFP as reporter gene.

The hybrid lac promoter (BBa_R0011), has been designed taking the Plambda promoter (BBa_R0051) and substituting its cI (BBa_C0051) binding sites with two lacI binding sites.

This promoter can be repressed by lacI (BBa_C0012), which can be repressed by lactose or IPTG. Differently from wild type lac promoter, this part does not have any CAP binding sites, so its behaviour is glucose-independent.

Strains bearing a genomic copy of lacI can repress this promoter, which acts as a glucose-independent lactose/IPTG sensor. In the other strains BBa_R0011 acts as a constitutive promoter.

Notes

We used BioBrick Standard Assembly.

Source

BBa_R0011, 2009 DNA Distribution;

BBa_E0240, 2009 DNA Distribution.

Components

Access	Instance	Definition
public	BBa_R0011	lacI+pL
public	BBa_I13507	BBa_I13507

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011	1,54	lacI+pL
BBa_I13507	64,924	BBa_I13507

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J107010/1

Member of these Collections

• iGEM Parts Registry