BBa_J107051

BBa_J107051 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J107051

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Giacomo Zambianchi

Date created: 2010-05-31 11:00:00

Date modified: 2015-08-31 04:08:25

Constitutive generator of Beta-Galactosidase + J23100

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_J107051_sequence (Version 1)

Description

This part consitutively expresses lacZ (BBa_I732005) under the control of Ptet strong promoter (BBa_R0040), which is always active because tetR gene (BBa_C0040) does not have any promoter upstream and so it cannot repress BBa_R0040.

If this part is contained in BBa_J61002 vector, RFP (BBa_E1010) is constitutively expressed by BBa_J23100 strong promoter.

This part confers the abilities of metabolizing lactose to lacZ mutant strains (e.g. TOP10 - BBa_V1009) and of glowing (when the vector is BBa_J61002), in order to (indirectly) detect bacterial growth by measuring RFP when the medium is turbid and the optical density (OD) cannot be effectively used as a cell count measure.

Notes

We used BioBrick Standard Assembly.

Source

BBa_K173006, UNIPV-Pavia iGEM Team;

BBa_J23100, 2009 DNA Distribution.

Components

Access	Instance	Definition
public	BBa_K173006	BBa_K173006
public	BBa_J23100	BBa_J23100

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K173006	1,4140	BBa_K173006
BBa_J23100	4149,4183	BBa_J23100

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J107051/1

Member of these Collections

• iGEM Parts Registry