BBa_J107054

BBa_J107054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J107054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lorenzo Pasotti

Date created: 2011-01-02 12:00:00

Date modified: 2015-08-31 04:08:25

adhB inducible system (aTc)

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_J107054_sequence (Version 1)

Description

This part is an aTc -> alcohol dehydrogenase II inducible system.

Alcohol dehydrogenase II enzyme (adhB gene, BBa_K173017) of Z. mobilis catalyzes the transformation of acetaldehyde into ethanol. This enzyme, as reported in literature, is also able to work in the opposite direction, oxidizing ethanol to acetaldehyde.

The expression of adhB in this part is constitutive in commonly used E. coli strains, but the promoter becomes inducible when the tetR protein is overexpressed, such as in DH5alpha-Z1, W3110-Z1 or MG1655-Z1 strains. In these strains, the promoter can be induced with aTc molecule.

Notes

We used BioBrick Standard Assembly

Source

Registry parts.

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_K173019	BBa_K173019

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_K173019	63,1375	BBa_K173019

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J107054/1

Member of these Collections

• iGEM Parts Registry