BBa_J119001

BBa_J119001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caleb J. Carr

Date created: 2011-07-20 11:00:00

Date modified: 2015-08-31 04:08:26

BsmBI-RBS-GFP1-BsaI

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_J119001_sequence (Version 1)

Description

First half of the full GFP gene for solving of HPP (Hamiltonian Path Problem) with multiple nodes. Works in conjunction with BBa_J119002 as second half of split GFP gene. The half edge noted below in the first 58nt of GFP-1/2 is intended to be annealed back to GFP-2/2 by JM109 causing green fluorescence. It is also intended to be mixed with other gene halves to attempt a multi-node HPP problem that will show correct completion if all reporters are present (resistance, fluorescence, etc.). All groupings must have both halves of a specific reporter gene to function properly.

GFP_1_For (58mer)
GCAT GAATTC CGTCTC A GTGG CA AAAGAGGAGAAA CGTACGAC ATGCGTAAAGGAGAA
4nt EcoRI BsmBI a 1234 nn RBS 0034 8nt 5mer of GFP1
(?? edge)

GFP_1_Rev (36mer)
ATGC CTGCAG GGTCTC A GGGT ATCACCTTCAAACTT
4 PstI BsaI a 1234 15mer of GFP_1 reverse
(Gene 1234 RC)

Notes

This is not a BioBrick part. It was designed and produced for the specific purpose of solving multi-node Hamiltonian Path Problems with the computing power of E Coli.

Source

PCR Amplification of the GFP template split with specific oligos to result in the full noted 407 nt sequence.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119001/1

Member of these Collections

• iGEM Parts Registry