Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-07-20 11:00:00
Date modified: 2015-08-31 04:08:26
BsaI-LacZ2-BsmbI
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J119004_sequence (Version 1) |
Description
Second half of the LacZ Gene (LacZ-2), split for use in HPP (Hamiltonian Path Problems). It is the necessary complement half of BBa_J119003, without the other, neither will function. They are intended to be re-assembled via transformation in E Coli JM109. It functions as a reporter gene when properly assembled with LacZ-1 by producing blue coloring (non-fluorescing) when X-Gal is present. Can be used in conjunction with other split genes but must have the complement to report properly.Beta-Gal_2_For (36mer)
5??? GCAT GAATTC GGTCTC A CCTG GAGAGGCGCGCCCGC 3???
(4, EcoRI , BsaI , a , Gene1234 , 15mer of Beta-Gal)
Beta-Gal_2_Rev (96mer)
5???ATGC CTGCAG CGTCTC A CCAC CCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACG TTGC TTATTTTTGACACCA 3???
(4 , PstI , BsmBI , a , Halfedge1234RC , pLacI rc , 4mer ,
15mer of Beta-Gal Rev)