Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-07-20 11:00:00
Date modified: 2015-08-31 04:08:26
BsmBI-RBS-RFP1-BsaI
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J119005_sequence (Version 1) |
Description
This is RFP-1, the first half of the RFP Gene Template to be used in conjunction with RFP-2 (J119006) to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a red fluorescence that may be visible under normal light conditions. Can be used with other split genes to solve more complex HPP problems.RFP_1_For (58mer)
5??? GCAT GAATTC CGTCTC A GTGG CA AAAGAGGAGAAA CGTACGAC ATGGCTTCCTCCGAA3???
(4, EcoRI, BsmBI , a , Halfedge1234 , nn , RBS 0034 , 8 , 15mer of RFP)
RFP_1_Rev (36mer)
5???ATGC CTGCAG GGTCTC A GCAG GGAGGAGTCCTGGGT 3???
(4, PstI , BsaI , a , Gene1234RC , 15mer of RFP reverse)
Notes
It was quite difficult to synthesize this half-edge (first half of the gene to be used in solving a path problem).This is NOT a BioBrick Part, it has been produced for the purpose of putting together and solving HPP (Hamiltonian Path Problems) by adding its second half (and others) to competent cells for re-assembling.