BBa_J119006

BBa_J119006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caleb J. Carr

Date created: 2011-07-20 11:00:00

Date modified: 2015-08-31 04:08:26

BsaI-RFP2-BsmBI

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J119006_sequence (Version 1)

Description

This is RFP-2, the second half of the RFP Gene Template to be used in conjunction with RFP-1 (J119005) to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a red fluorescence that may be visible under normal light conditions. Can be used with other split genes to solve more complex HPP problems.

RFP_2_For (36mer)

5??? GCAT GAATTC GGTCTC A CTGC AAGACGGTGAGTTCA 3???

(4, EcoRI , BsaI, a , Gene1234 , 15mer of RFP)

RFP_2_Rev (75mer)

5???ATGC CTGCAG CGTCTC A CCAC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA TTGC TTATTAAGCACCGGT 3???

(4, PstI , BsmBI , a , Halfedge1234RC , J23100 Reverse Complement , 4 , 15mer of RFP Rev)

Notes

This is NOT a BioBrick Part, it has been produced for the purpose of putting together and solving HPP (Hamiltonian Path Problems) by adding its second half (and others) to competent cells for re-assembling.

Source

PCR amplification of the RFP gene template.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119006/1

Member of these Collections

• iGEM Parts Registry