| Types | DnaRegion
|
| Roles | sequence_feature
DNA
|
| Sequences | BBa_J119007_sequence (Version 1)
|
Description
Vector for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. EcoRI site on the left. Then there is a J23100 promoter that points to the right. It is followed by a half-edge sticky word of GTGG, made available by a BsmBI site pointing to the left. Next is a BsaI site pointing to the right that frees up the following half-edge sticky word of GTGG. Finally, there is a PstI site.
Notes
Standard BioBrick prefix and suffix are not used. Only a EcoRI site is used as a prefix and a Pst I site as a suffix.
Source
Cloned from synthetic oligonucleotides into pSB1A2 EcoRI + PstI vector.
