Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Barta
Date created: 2011-07-26 11:00:00
Date modified: 2015-08-31 04:08:26
pLacI-RBS-T7RNAP with sites for oligo insertion
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J119009_sequence (Version 1) |
Description
J23100 promoter and B0030 RBS upstream of T7 RNAP gene. The following restriction sites do not occur in the promoter-RBS-T7 RNAP sequence. Some of them did not occur in the original sequence and others were removed while conserving the amino acid sequence. Both the forward and reverse complement were checked for type IIs enzyme sites. EcoRI, XbaI, SpeI, PstI, AscI, AsiSI, BsaI, BsmBI, BbsINotes
BsmBI site (cgtctcn/ and /nnnnngagacg) before RBS and after gene coding sequence that can be used to cut out RBS-gene for placement after other promoters. Sites for AsiSI (gcgat/cgc) and AscI (gg/cgcgcc) that can be used to insert existing oligos. Sites flanking these for BsaI (ggtctcn/ and /nnnnngagacc) that can be used to insert new oligos without the need for gel purification. A site for BbsI (gaagacnn/ and /nnnnnngtcttc) that produces a sticky end complementary to the first BsaI site, for use in generating a near wild type version of the gene.Source
GeneArt| Sequence Annotation | Location | Component / Role(s) |
| pLacI BsmBI B0030 Start RNAP BsaI AsisI Asc1 BsaI BbsI BsmBI Stop RNAP T7 RNA Polymerase | 1,56 57,62 68,82 89,91 99,104 107,114 117,124 126,131 137,142 2800,2805 2789,2791 89,2788 | promoter feature/promoter feature/misc sequence_feature feature/rbs ribosome_entry_site start_codon feature/start sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature stop_codon feature/stop CDS feature/cds |