Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zachary Caton
Date created: 2011-08-02 11:00:00
Date modified: 2015-08-31 04:08:26
BsaI-GFP2 (BsaI removed)-BsmBI
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J119011_sequence (Version 1) |
Description
This is GFP-2A, the second half of the GFP Gene Template to be used in conjunction with RFP-1 (J119005) to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a red fluorescence that may be visible under normal light conditions. Can be used with other split genes to solve more complex HPP problems.Notes
This is NOT a BioBrick compatible part. It was designed to solve Hamiltonian Path Problems using multiple genes.There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site.
Source
PCR Amplification of existing part: GFP (E0040)split 351 bp from the beginning of the gene at with the sticky word ACCC| Sequence Annotation | Location | Component / Role(s) |
| Sticky Word: ACCC BsaI EcoRI Rogue BsaI site Sticky Word: GCAA BsmBI Sticky Word: GTGG PstI | 14,17 7,13 2,6 305,311 383,386 445,451 441,444 452,457 | feature/misc sequence_feature non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/misc sequence_feature non_covalent_binding_site feature/binding sequence_feature feature/misc feature/binding non_covalent_binding_site |