BBa_J119013

BBa_J119013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-08-17 11:00:00
Date modified: 2015-08-31 04:08:26

FINISH



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_J119013_sequence (Version 1)

Description

Finish half edge for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. BsmBI site pointing right that when digested frees up half edge word "GTGG" on the 5' end that allows ligation to any gene 2 half-edge. Followed by the BsaI site pointing left that when digested frees up the FINISH word "CGAG" that allows ligation to the new tr5f4HPP vector. In between these two sites is an 8 nt spacer. This section is flanked by EcoRI and PstI sticky ends for cloning.

Notes

Half-edge words must match the half edge words exactly that you want to ligate to.

Source

Oligo constructs designed for the purpose of solving Hamiltonian Path graphs.

Sequence Annotation Location Component / Role(s)
EcoRI
BsmBI
8 NT Spacer
Finish Word
Half-Edge Word
BsaI
PstI
1,6
7,12
17,24
25,28
13,16
29,34
35,40
sequence_feature feature/dna
sequence_feature feature/dna
sequence_feature feature/dna
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/dna
feature/dna sequence_feature
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119013/1