Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-08-17 11:00:00
Date modified: 2015-08-31 04:08:26
HPP assembly vector
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J119014_sequence (Version 1) |
Description
New HPP vector for use in Golden Gate Assembly of Hamiltonian Path Problem constructs.EcoRI site followed by the START sticky word made available by the following BsaI site pointing left. Followed by NheI, then another BsaI site pointing right that frees up the FINISH sticky word. Followed by the PstI site.
Notes
1. Use BsaI to free up a new START word and a new FINISH word that are different from any of the Gene words (listed below).2. NheI site in the middle for cloning in any XbaI/SpeI BioBrick fragment, such as the ccdB Death Gene or a reporter expression cassette.
3. Flanked by EcoRI and PstI sticky ends.
Source
HPP Vector Oligo designed for use in HPP solutions.| Sequence Annotation | Location | Component / Role(s) |
| EcoRI START word BsaI NheI BsaI FINISH word PstI | 1,6 7,10 11,16 17,22 23,28 29,32 33,38 | sequence_feature feature/dna feature/misc sequence_feature sequence_feature feature/dna feature/dna sequence_feature feature/dna sequence_feature feature/misc sequence_feature feature/dna sequence_feature |