Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-10-18 11:00:00
Date modified: 2015-08-31 04:08:26
HPP Bsa J23100 promoter in pSB1A2
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J119022_sequence (Version 1) |
Description
J23100 promoter in pSB1A2 for BsaI/Ligase insertion into pSB111 (J100028).This is the J23100 promoter fragment designed for BsaI digestion and ligation into pSB111 vector (part #J100028)for use in Golden Gate Assembly of Hamiltonian Path Problem constructs.
It is in pSB1A2 with a BsaI site on the left reading right, with word CGAC being freed up, followed by J23100 promoter, followed up by word GCGG which is freed up by the following BsaI site which reads to the left. The fragment is 57nt long after digestion. It should then be ligated into vector pSB111 (part J100028).
Notes
Sticky ends must match only the destination sites and none else. Otherwise part will not work. Otherwise, J23100 is a common promoter, but the fore and aft tails must be unique to function properly.Source
J23100 promoter with added sticky ends due to digestion with BsaI for insert into a destination vector for HPP construct problems.| Sequence Annotation | Location | Component / Role(s) |
| 1 bp BsaI cuts right left sticky end CGAC J23100 right sticky end GCGG 1 bp BsaI cuts left Vector Vector | 28,28 22,27 29,32 33,68 69,72 73,73 74,79 1,21 80,98 | sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/dna feature/promoter promoter sequence_feature feature/dna sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/dna sequence_feature feature/dna |