BBa_J119025

BBa_J119025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Vivek Mutalik

Date created: 2011-12-21 12:00:00

Date modified: 2015-10-01 11:42:55

BD2 bicistronic translational junction - Lowest efficiency

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_J119025_sequence (Version 1)

Description

The BD2 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest.

Notes

The bicistronic translational junction was designed by Team Leader Vivek Mutalik at BioFAB. It is is intended to give reproducible and consistent expression for various genes of interest.

Source

Synthetic DNA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
leader RBS	19,27	ribosome_entry_site, feature/rbs
Start	33,35	feature/start, start_codon
Start	86,88	start_codon, feature/start
Stop	84,86	stop_codon, feature/stop
GOI RBS	71,79	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119025/1

Member of these Collections

• iGEM Parts Registry