Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl and A. Malcolm Campbell
Date created: 2012-01-04 12:00:00
Date modified: 2015-08-31 04:08:26
pSB1A8
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_J119043_sequence (Version 1) |
Description
Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.Notes
BsaI site removed from amplicillin resistance gene.Source
De novo DNA synthesis.| Sequence Annotation | Location | Component / Role(s) |
| suffix prefix B0025 terminator B1010 terminator VR primer VF2 primer pMB1 replication origin (rev) Amp Resistance (rev) stuffer mutation A --> G removes BsaI site | 1,21 2207,2228 22,205 2167,2206 208,227 2069,2088 310,923 1085,1946 2228,2240 1227,1227 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/misc sequence_feature feature/misc sequence_feature feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site sequence_feature feature/dna feature/cds CDS feature/misc sequence_feature feature/s_mutation point_mutation |