BBa_J119045

BBa_J119045 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119045

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl and A. Malcolm Campbell

Date created: 2012-01-19 12:00:00

Date modified: 2015-08-31 04:08:26

pSB1C8

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J119045_sequence (Version 1)

Description

Insulated vector based on pSB1C3. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.

Notes

Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de novo DNA synthesis.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Prefix	2134,2155	feature/BioBrick, engineered_region
Suffix	1,21	feature/BioBrick, engineered_region
VR primer	208,227	primer_binding_site, feature/primer_binding
VF2	1984,2003	primer_binding_site, feature/primer_binding
Chloramphenicol resistance	1213,1872	CDS, feature/cds
pMB1 replication origin	313,927	sequence_feature, feature/misc
B0025 terminator	22,205	feature/misc, sequence_feature
B0010 terminator	2094,2133	feature/misc, sequence_feature
stuffer	2155,2167	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119045/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors