BBa_J119046

BBa_J119046 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119046

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: A. Malcolm Campbell and Todd Eckdahl

Date created: 2012-01-23 12:00:00

Date modified: 2015-08-31 04:08:26

pSB1K8

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J119046_sequence (Version 1)

Description

Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.

Notes

Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de nove DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	engineered_region, feature/BioBrick
Prefix	2256,2277	feature/BioBrick, engineered_region
VR primer	208,227	primer_binding_site, feature/primer_binding
VF2 primer	2118,2137	primer_binding_site, feature/primer_binding
B0025 terminator	22,205	feature/stem_loop, stem_loop
B0010 terminator	2138,2215	stem_loop, feature/stem_loop
stuffer	2278,2289	feature/misc, sequence_feature
pMB1 replication origin	310,923	sequence_feature, feature/misc
Kanamycin resistance gene	1148,1963	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_J119046/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors