BBa_J119048

BBa_J119048 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119048

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: A. Malcolm Campbell and Todd Eckdahl

Date created: 2012-01-23 12:00:00

Date modified: 2015-08-31 04:08:26

pSB4A8

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J119048_sequence (Version 1)

Description

Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.

Notes

BsaI site removed from amplicillin resistance gene.
Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de novo DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	feature/BioBrick, engineered_region
Prefix	3629,3650	feature/BioBrick, engineered_region
VR primer	208,227	primer_binding_site, feature/primer_binding
VF2 primer	3491,3510	primer_binding_site, feature/primer_binding
Ampicillin resistance gene	2508,3368	CDS, feature/cds
I50042 low copy pSC101 origin	314,2350	sequence_feature, feature/misc
B0025 terminator	22,205	feature/stem_loop, stem_loop
B0010	3589,3628	feature/stem_loop, stem_loop
stuffer	3651,3662	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119048/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors