BBa_J119049

BBa_J119049 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119049

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: A. Malcolm Campbell and Todd Eckdahl

Date created: 2012-01-23 12:00:00

Date modified: 2015-08-31 04:08:26

pSB4C8

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J119049_sequence (Version 1)

Description

Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.

Notes

Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de novo DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Prefix	3544,3565	feature/BioBrick, engineered_region
Suffix	1,21	engineered_region, feature/BioBrick
stuffer	3566,3577	sequence_feature, feature/misc
VF2 primer	3406,3425	feature/primer_binding, primer_binding_site
VR primer	208,227	feature/s_mutation, point_mutation
B0025 terminator	22,205	feature/stem_loop, stem_loop
B0010 terminator	3504,3543	feature/stem_loop, stem_loop
I50042 low copy pSC101 origin	314,2350	feature/misc, sequence_feature
Chloramphenicol resistance gene	2633,3294	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119049/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors