BBa_J119050

BBa_J119050 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119050

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl and A. Malcolm Campbell

Date created: 2012-01-23 12:00:00

Date modified: 2015-08-31 04:08:26

pSB4K8

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J119050_sequence (Version 1)

Description

Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.

Notes

Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

de novo DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	engineered_region, feature/BioBrick
Prefix	3679,3700	engineered_region, feature/BioBrick
stuffer	3701,3712	sequence_feature, feature/misc
VF2 primer	3541,3560	feature/primer_binding, primer_binding_site
VR primer	208,227	feature/primer_binding, primer_binding_site
B0025 terminator	22,205	feature/stem_loop, stem_loop
B0010 terminator	3561,3638	stem_loop, feature/stem_loop
I50042 low copy pSC101 origin	310,2346	sequence_feature, feature/misc
Kanamycin resistance gene	2571,3386	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119050/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors