Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sachii Polpitiya Arachchige
Date created: 2013-07-01 11:00:00
Date modified: 2015-08-31 04:08:28
RFP expression cassette for J-GGA
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_J119306_sequence (Version 1) |
Description
The scaffold for Junction Golden Gate Assembly (J-GGA) is populated with a promoter related to P5 (J110031), a C dog RBS element related to BD2 (J119025), and a mRFP codon optimized for E. coli. J-GGA can be used to vary any of the elements. Inverted PCR from two junctions is followed by BsaI/Ligase assembly of elements carrying the appropriate BsaI sites or sticky ends.Notes
None.Source
Constructed by J-GGA from scaffold.| Sequence Annotation | Location | Component / Role(s) |
| Junction B Junction C Junction A Junction D mRFP E coli Promoter C dog RBS | 54,71 151,168 1,18 847,864 166,846 19,53 72,150 | feature/dna sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/dna sequence_feature CDS feature/cds promoter feature/promoter feature/rbs ribosome_entry_site |