Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Claire Shinneman, Paul Frederick, Jason Yuan
Date created: 2013-09-07 11:00:00
Date modified: 2015-08-31 04:08:28
pClone Blue - Device for Testing New Promoters via Golden Gate Assembly
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J119313_sequence (Version 1) |
Description
The scaffold consist of four junctions, which allow to insert parts(promoters.RBS,Genes)between junctions A and Junction B,Junction B and Junction C or the Junction C and Junction DNotes
N/ASource
N/A| Sequence Annotation | Location | Component / Role(s) |
| GFP reverse RBS reverse CGAC Sticky end GCGG Sticky end BsaI left BsaI right pLacIQ1 reverse BD18 bicistron RBS AmilCP Blue double stop | 1,720 727,741 755,758 844,847 760,765 837,842 766,836 861,947 945,1613 1608,1613 | CDS feature/cds feature/rbs ribosome_entry_site feature/misc sequence_feature sequence_feature feature/misc feature/dna sequence_feature sequence_feature feature/dna feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds feature/stop stop_codon |