Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2013-10-04 11:00:00
Date modified: 2015-08-31 04:08:28
Scaffold 2.0 for J-GGA
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J119314_sequence (Version 1) |
Description
Scaffold 2.0 allows the use of BsaI Junction Golden Gate Assembly (J-GGA) to build a gene expression cassette. The vector is prepared by inverted PCR (iPCR) using primers with BsaI sites that produce 5' overhanging sticky ends for the junctions involved. The insert either has the same sticky ends or carries BsaI sites that produce them. J-GGA is then performed with BsaI and T4 DNA Ligase.Notes
Scaffold 2.0 was designed by GGAJET (http://gcat.davidson.edu/SynBio13/GGAJET/).Source
Synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| Junction A Junction B Junction C Junction A (3' sticky end) Junction B (5' sticky end) Junction B (3' sticky end) Junction C (5' Sticky end) Junction C (3 Junction D (5' sticky end) Junction D Junction D (3' sticky end) Junction A (5' sticky end) | 22,39 44,61 66,83 37,40 44,47 57,60 66,69 79,82 88,91 88,102 102,105 22,25 | sequence_feature feature/dna feature/dna sequence_feature feature/dna sequence_feature sequence_feature feature/dna feature/dna sequence_feature sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/dna feature/dna sequence_feature feature/dna sequence_feature feature/dna sequence_feature |