Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2013-10-04 11:00:00
Date modified: 2015-08-31 04:08:28
Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J119315_sequence (Version 1) |
Description
Scaffold 2.0 with the promoter in the junction A.The vector is prepared by inverted PCR (iPCR) using primers with BsaI sites that produce 5' overhanging sticky ends for the junctions A and Junction B. The promoter has the same sticky ends.J-GGA is then performed with BsaI and T4 DNA Ligase.Notes
N/ASource
Synthesized Oligos| Sequence Annotation | Location | Component / Role(s) |
| P5 highest J119031 Junction A Junction B Junction C Junction D | 39,75 22,39 76,98 98,114 119,137 | feature/promoter promoter sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/dna feature/dna sequence_feature |