Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2013-10-15 11:00:00
Date modified: 2015-08-31 04:08:28
Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J119316_sequence (Version 1) |
Description
Scaffold 2.0 with the RBS in the junction B.The vector is prepared by inverted PCR (iPCR) using primers with BsaI sites that produce 5' overhanging sticky ends for the junctions B and Junction C. The RBS has the same sticky ends.J-GGA is then performed with BsaI and T4 DNA Ligase.Notes
N/ASource
N/A| Sequence Annotation | Location | Component / Role(s) |
| Promoter (P5) RBS(BD18) Junction C Junction D | 39,74 94,172 173,189 195,212 | feature/promoter promoter feature/rbs ribosome_entry_site sequence_feature feature/dna sequence_feature feature/dna |