Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2013 Tuesday 2:00 lab
Date created: 2013-11-18 12:00:00
Date modified: 2015-08-31 04:08:28
pTac mutant -8 AA deletion
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J119324_sequence (Version 1) |
Description
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2013. It was cloned into the pClone Green vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation is an insertion of CCA at position -12. The mutation had a large negative effect on the function of the promoter (see Experience page).Notes
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.Source
Synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| -35 region AA deleted | 7,12 31,31 | feature/promoter promoter sequence_alteration feature/mutation |