Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2013 Thursday 8:00 lab
Date created: 2013-11-18 12:00:00
Date modified: 2015-08-31 04:08:28
pTac mutant -10 TTTTT
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J119326_sequence (Version 1) |
Description
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2013. It was cloned into the pClone Green vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation converts the -10 region from TATAAT to TTTTTT. The mutation had a large negative effect on the function of the promoter (see Experience page).Notes
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.Source
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.| Sequence Annotation | Location | Component / Role(s) |
| -35 region mutant -10 region | 7,12 29,34 | feature/promoter promoter sequence_alteration feature/mutation |