BBa_J119332

BBa_J119332 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119332
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-02-03 12:00:00
Date modified: 2015-08-31 04:08:28

Ammeline Biosensor with Riboswitch M6 + RFP



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_J119332_sequence (Version 1)

Description

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.

Notes

None.

Source

Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly.

Sequence Annotation Location Component / Role(s)
P5 promoter
RFP
M6 Ammeline riboswitch
19,55
183,866
73,188
promoter feature/promoter
feature/cds CDS
feature/rbs ribosome_entry_site
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_J119332/1