BBa_J119349

BBa_J119349 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119349

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kelly Cochran

Date created: 2014-06-29 11:00:00

Date modified: 2015-08-31 04:08:28

Ammeline Biosensor with Riboswitch D2 + RFP

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_J119349_sequence (Version 1)

Description

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (D2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The D2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and the sequestration sequence in the P2 stem of the aptamer (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).

Notes

The only differences between the M6C" riboswitch and D2 is the RBS and sequestration sequence location.

Source

Riboswitch sequence TCAACACTTCATTTAATCCCGTGTATATGGTTTCGGAGCTTCCACCAAGAGCCTTAAACTCTTGACTATGAAGTCTGTCGCTTTATCCGAAATTTTATACACAGGATTGACTATGAAG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 promoter	19,55	feature/promoter, promoter
D2 Ammeline riboswitch	73,190	feature/rbs, ribosome_entry_site
RFP	185,868	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119349/1

Member of these Collections

• iGEM Parts Registry