Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kelly Cochran
Date created: 2014-07-06 11:00:00
Date modified: 2015-08-31 04:08:28
Ammeline Biosensor with Riboswitch N2 + RFP
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J119352_sequence (Version 1) |
Description
The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).Notes
The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).Source
M6C"| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter N2 Ammeline riboswitch RFP | 19,55 73,190 185,868 | promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS |