Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2014 Tuesday 8:00 lab
Date created: 2014-11-07 12:00:00
Date modified: 2015-08-31 04:08:28
Ptac mutant -40 G Deletion
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J119368_sequence (Version 1) |
Description
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2014. It was cloned into the pClone Red vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation deletes the G at position -40. The mutation had a minimal effect on the function of the promoter (see Experience page).Notes
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.Source
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.| Sequence Annotation | Location | Component / Role(s) |
| -40 G deletion -10 region -35 region | 1,1 28,33 6,11 | feature/mutation sequence_alteration promoter feature/promoter feature/promoter promoter |