Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2014 Tuesday 8:00 lab
Date created: 2014-11-07 12:00:00
Date modified: 2015-08-31 04:08:28
Ptac mutant -28 T Insertion
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J119369_sequence (Version 1) |
Description
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2014. It was cloned into the pClone Red vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation inserts a T at position -28. The mutation had a minimal effect on the function of the promoter (see Experience page).Notes
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.Source
Synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| T insertion -10 region -35 region | 13,13 30,35 7,12 | feature/mutation sequence_alteration promoter feature/promoter feature/promoter promoter |