Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2014 Thursday 8:00 lab
Date created: 2014-11-07 12:00:00
Date modified: 2015-08-31 04:08:28
Ptac mutant -10 Region Shifted Upstream 7 bp
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J119373_sequence (Version 1) |
Description
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2014. It was cloned into the pClone Red vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation shifts the -10 region upstream 7 bp. The mutation had a large effect on the function of the promoter (see Experience page).Notes
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.Source
Synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| insertion of -10 -35 region deletion of -10 | 22,27 7,12 35,40 | feature/mutation sequence_alteration feature/promoter promoter feature/mutation sequence_alteration |