Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Camille Johnson, Helen Webster, Sean Holloran
Date created: 2014-11-13 12:00:00
Date modified: 2015-08-31 04:08:28
tClone TetA: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_J119374_sequence (Version 1) |
Description
tClone is a construction intermediate that can be used to build devices that will allow users to clone and test new transcriptional terminators and riboswitches that function by antitermination without gel purification or other preparation of DNA. Use of BioBrick cloning to add the TetA gene into the suffix of tClone produce tClone TetA, which can be used to measure new transcriptional terminators and riboswitches. tClone vectors can be used as destination vectors for Golden Gate Assembly (GGA) using BsaI and ligase. A new terminator or riboswitch can be derived from synthetic oligos, PCR, or a plasmid clone. For proper assembly, see BBa_J119367. Whether or not transcription proceeds to the TetA gene is determined by the strength of the terminator or whether termination or antitermination occurs at the riboswitch. The part incorporates the BD18 bicistronic translational junction (see Part:BBa_J119024) engineered by Vivek Mutalik and The BIOFAB Team at biofab.org.Notes
J119367+S05245Source
J119367+S05245| Sequence Annotation | Location | Component / Role(s) |
| P5 Promoter BsaI Left GFP B0030 RBS rev P2 Promoter rev BsaI Right TetA BD18 C Dog RBS | 1,36 42,47 55,774 781,792 803,848 849,854 946,2142 861,946 | promoter feature/promoter feature/binding non_covalent_binding_site feature/cds CDS feature/rbs ribosome_entry_site promoter feature/promoter non_covalent_binding_site feature/binding CDS feature/cds ribosome_entry_site feature/rbs |