Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Eckdahl
Date created: 2015-02-26 12:00:00
Date modified: 2015-08-31 04:08:28
81 Mutated Ptac Promoters with Varying Strengths
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J119375_sequence (Version 1) |
Description
Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry.Notes
None.Source
Synthetic oligonucleotides were cloned into pClone Red using BsaI Golden Gate Assembly.| Sequence Annotation | Location | Component / Role(s) |
| -10 region -35 region NNNNNN | 33,38 11,16 11,16 | promoter feature/promoter feature/promoter promoter feature/mutation sequence_alteration |