| Types || |
| Roles |
| Sequences || BBa_J119390_sequence (Version 1)|
DescriptionOligonucleotides that encoded RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384)to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
NotesThe oligos needed to include a 5' sticky end of CGAC and a 3'sticky end of CCGC to ligate into rClone Red with BsaI Golden Gate Assembly.
SourceSynthetic oligos cloned into rClone Red (BBa_J119384).