| Types | DnaRegion
|
| Roles | ribosome_entry_site
RBS
|
| Sequences | BBa_J119393_sequence (Version 1)
|
Description
Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (
BBa_J119384). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
Notes
The oligos needed to include a 5' sticky end of CGAC and a 3'sticky end of CCGC to ligate into rClone Red with BsaI Golden Gate Assembly.
Source
Synthetic oligos cloned into rClone Red (BBa_J119384).
