Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Athena J. Davis
Date created: 2016-06-23 11:00:00
Date modified: 2016-06-24 09:35:06
SacB + PDB riboswitch
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J119396_sequence (Version 1) |
Description
The PDB riboswitch consists of a theophylline aptamer sequence from the Protein Databank. This aptamer sequence was inserted into the Wachsmuth et al. RS3 riboswitch sequence using Golden Gate Assembly. The SacB plasmid backbone was obtained from part J119042, and Golden Gate Assembly was used to ligate the riboswitch into the SacB plasmid.Notes
NoneSource
Existing part BBa_J119042 and annealed oligos containing the PDB riboswitch sequence.| Sequence Annotation | Location | Component / Role(s) |
| pTet BD18 bicistronic junction SacB PDB riboswitch | 1,52 127,214 212,1636 55,126 | sequence_feature feature/misc feature/rbs ribosome_entry_site feature/cds CDS feature/stem_loop stem_loop |