BBa_J119397

BBa_J119397 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119397
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hunn
Date created: 2016-07-05 11:00:00
Date modified: 2016-07-06 10:34:43

pJC173b gIII neg (Carlson et al. 2014)



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_J119397_sequence (Version 1)

Description

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat. This construct is based on J100265 and contains a deletion of 957 nt, encoding 319 amino acids, from gene III. The mutant gene III encodes a protein III that acts as a dominant negative for M13 phage reproduction.

Notes

No BbsI sites in J100265 plasmid.

Source

iPCR of J100265 plasmid with primers that introduce BbsI site for Golden Gate Assembly.

Sequence Annotation Location Component / Role(s)
RepA
SC101 Origin of Replication
T7
T7 Promoter
SD8 RBS
geneIII neg
LuxAB
AmpR
bla promoter
1,951
955,1700
1785,1804
1785,1804
1826,1840
1841,2162
2158,4281
4361,5221
5222,5416
CDS feature/cds
sequence_feature feature/misc
feature/primer_binding primer_binding_site
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/cds CDS
CDS feature/cds
CDS feature/cds
promoter feature/promoter
igem#experience None  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_J119397/1