Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hunn
Date created: 2016-07-05 11:00:00
Date modified: 2016-07-06 10:34:43
pJC173b gIII neg (Carlson et al. 2014)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J119397_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat. This construct is based on J100265 and contains a deletion of 957 nt, encoding 319 amino acids, from gene III. The mutant gene III encodes a protein III that acts as a dominant negative for M13 phage reproduction.Notes
No BbsI sites in J100265 plasmid.Source
iPCR of J100265 plasmid with primers that introduce BbsI site for Golden Gate Assembly.| Sequence Annotation | Location | Component / Role(s) |
| RepA SC101 Origin of Replication T7 T7 Promoter SD8 RBS geneIII neg LuxAB AmpR bla promoter | 1,951 955,1700 1785,1804 1785,1804 1826,1840 1841,2162 2158,4281 4361,5221 5222,5416 | CDS feature/cds sequence_feature feature/misc feature/primer_binding primer_binding_site promoter feature/promoter feature/rbs ribosome_entry_site feature/cds CDS CDS feature/cds CDS feature/cds promoter feature/promoter |