BBa_J119400

BBa_J119400 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119400

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Avery Umphreys

Date created: 2016-07-17 11:00:00

Date modified: 2016-07-18 09:22:25

tCloneTet+PDB riboswitch with P5 and BD10

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_J119400_sequence (Version 1)

Description

The BD10 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD10 was inserted via Decoupled Golden Gate Assembly.

Notes

BD10 was inserted via Decoupled Golden Gate Assembly. iPCR amplified everything on the J100255 plasmid, besides BD18.

Source

J100255 is the original tClone Tet+PDB Riboswitch and it is from Campbell M Lab. J119026, located in Eckdahl Lab, includes the BD10 C Dog RBS.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
p5	102,137	promoter, feature/promoter
BD10	222,305	ribosome_entry_site, feature/rbs
TetA	307,726	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119400/1

Member of these Collections

• iGEM Parts Registry