BBa_J119402

BBa_J119402 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119402

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony J. Eckdahl

Date created: 2016-07-31 11:00:00

Date modified: 2016-08-01 01:50:06

Double NiCo Riboswitch in tClone Red

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_J119402_sequence (Version 1)

Description

A riboswitch found to be responsive to transitions metals (Ni++, Co++, Mn++) from Furakawa et al. (Molecular Cell 57: 1088-1098, 2015) was cloned as a tandem repeat into tClone Red using BsaI Golden Gate Assembly.

Notes

No internal BsaI sites.

Source

Synthetic oligonucleotides.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 promoter	1,36	promoter, feature/promoter
NiCo Riboswitch	41,167	feature/stem_loop, stem_loop
NiCo Riboswitch	168,294	feature/stem_loop, stem_loop
BD18 C dog RBS	299,383	feature/rbs, ribosome_entry_site
RFP	384,1064	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119402/1

Member of these Collections

• iGEM Parts Registry