Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony J. Eckdahl
Date created: 2016-07-31 11:00:00
Date modified: 2016-08-01 01:57:40
Triple NiCo Riboswitch in tClone Red
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J119403_sequence (Version 1) |
Description
A riboswitch found to be responsive to transitions metals (Ni++, Co++, Mn++) from Furakawa et al. (Molecular Cell 57: 1088-1098, 2015) was cloned as three direct repeats into tClone Red using BsaI Golden Gate Assembly.Notes
No internal BsaI sites.Source
Synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| NiCo Riboswitch NiCo Riboswitch BD18 C dog RBS NiCo Riboswitch RFP P5 promoter | 41,167 168,294 426,510 295,421 511,1191 1,36 | feature/stem_loop stem_loop stem_loop feature/stem_loop feature/rbs ribosome_entry_site feature/stem_loop stem_loop CDS feature/cds feature/promoter promoter |