Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lindsay Doolan and the fall 2016 Missouri Western Genetics class
Date created: 2017-03-24 12:00:00
Date modified: 2017-03-24 11:53:56
(pSB1A2-BR) Deletion of the original -35 sequence
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J119405_sequence (Version 1) |
Description
The Pupp promoter responds to UTP (see BBa_J100305). This mutant has a deletion of the original -35 element (TGCCGC) to test the effects on transcription with a different -35 sequence (AAGAAT).Notes
The original -35 region, was deleted, but transcription was not terminated, the sequence just shifted, filling in a new sequence for the -35 region.Source
Part-sequence only (35 bp)AAGAATACCTTGAAGAAAGGAGGTATAATCCGTCG
| Sequence Annotation | Location | Component / Role(s) |
| Pupp-35 element deletion of the -35 region | 8,13 1,5 | promoter feature/promoter sequence_alteration feature/mutation |