BBa_J119407

BBa_J119407 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119407
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christian Mackley and the fall 2016 Missouri Western State University Genetics class
Date created: 2017-02-15 12:00:00
Date modified: 2017-02-21 12:32:58

Pupp promoter mutant - Deletion of -10 element



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J119407_sequence (Version 1)

Description

The Pupp promoter responds to UTP (see BBa_J100305). This mutant has a deletion of the original -10 element (TATAAT) to test the effects on transcription with a different -10 sequence (AGGAGG).

Notes

None

Source

Synthetic oligonucleotides cloned into pClone Red (BBa_J119137).

Sequence Annotation Location Component / Role(s)
Pupp -35 element
Deletion of -10 element
8,13
29,30
feature/promoter promoter
feature/mutation sequence_alteration
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_J119407/1